UNIVERSITY OF MASSACHUSETTS
The broad goal of this work is to use the C. elegans model system to understand the genetic and molecular mechanisms underlying the developmental regulation of microRNA expression. There are more than 150 distinct microRNA genes in C. elegans, including more than 20 families of microRNAs with homologs in humans. The functions of these are largely unknown, although it is clear that microRNAs play important roles in a wide spectrum of developmeental and phsyiologocal processes in all animals. Little is known about how microRNA gene expression is regulated transcriptionaly or post-transcriptionally by developmental and physiologiical signals. Molecular and genetic approaches will be employed to characterize mechanisms of microRNA transcriptional regulation in C.elegans, and to characterize the proteins and RNA elements responsible for post-transcriptional developmental regulation of microRNA biogenesis.
| AWARD OVERVIEW |
| Award Number |
1R01Gm088365-01 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$771,298 |
Project Location - City |
Worcester |
| Award Date |
09/17/2009 |
Project Location - State |
MA |
| Project Status |
Completed |
Project Location - Zip |
01655-0002
|
| Jobs Reported |
2.90 |
Congressional District |
03 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
UNIVERSITY OF MASSACHUSETTS |
| Recipient DUNS Number |
603847393
|
| Recipient Address |
55 LAKE AVE N |
| Recipient City |
WORCESTER |
| Recipient State |
Massachusetts |
| Recipient Zip |
01655-0002 |
| Recipient Congressional District |
03 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
Developmental Regulation of MicroRNA Expression |
| Project Status |
Completed |
| Final Project Report Submitted |
Yes |
| Project Activities Description |
Medical Research, General/Other |
| Quarterly Activities/Project Description |
In this quarter we completed testing constructs containing segments of the lin-4 promotor that had been previously refractory to yeast 1-hybrid assays. For the other component of the project, exploring post-transcriptional regulation of microRNA gene expression, we generated a set of transgene constructs that are designed to delineate the sequences and structures surrounding the mir-35-41 cluster. We have these sequences contained within and close to the microRNA precursor that confer stage-specificity of processing. We identified sequences that confer normal developmental patterns of processing, and have intitiated more refined constructs designed to tests loop and stem sequences of the mir-35 microRNA. We continued to make progress in developing reporter transgenes for assay microRNA levels in vivo for the purpose of screening for mutants defective in the normal regulation of microRNA levels during development. We have established assay conditions for these screens and initated pilot screens to evaluate feasibility of the approach. |
| Jobs Created |
2.90 |
| Description of Jobs Created |
Assoc Professor, Bioinformatician II, 2 Post Doc Assocs, Professor, Research Scientist, 2 UMass Graduate Students |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0852 |
| Award Information |
| Award Date |
09/17/2009 |
| Award Number |
1R01Gm088365-01 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$771,298 |
| Funds Invoiced/Received |
$770,983 |
| Expenditure Amount |
$771,298 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
H01 |
| Activity Description |
Medical Research, General/Other |
| Sub-Awards Information |
| Sub-awards to Organizations |
0 |
| Sub-award Amounts to Organizations |
$0 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
0 |
| Total Amount of payments to vendors greater than $25,000/award |
$0 |
| Number of payments to vendors less than $25,000/award |
174 |
| Total Amount of payments to vendors less than $25,000/award |
$25,697 |
| Location Information |
| Latitude, Longitude |
42º 16' 39",
-71º 45' 33" |
| Congressional District |
03 |
| Address 1 |
55 Lake Avenue North |
| Address 2 |
|
| City |
Worcester |
| County |
Worcester |
| State |
MA |
| Zip |
01655-0002 |
|
|