REGENTS OF THE UNIVERSITY OF MINNESOTA
This project occurred at a subcontract site, Gustavus University.
The goal of this project is to give undergraduate students research experience to attract them into science. The ability to engage students in thinking about solving problems of real biomedical significance contributes to a deeper understanding of modern challenges in analytical chemistry, and provides the opportunity to talk with them about potential careers in analytical chemistry, medicine, and opportunities at the interface between these fields. The research project is to develop a low cost analytical methodology for rapid, accurate, and precise quantitation of target biomarkers found in human urine. Previously we identified the molecule desmosine as a high value target for the development of novel analytical methodology. Last summer we developed a two-dimensional separation approach based on high performance liquid chromatography. During our work we quickly learned about some of the challenges associated with quantitation of low-abundance compounds in a complex fluid such as human urine. Indeed a significant portion of our time was spent trying to understand interactions between desmosine and other constituents of the urine sample.