UNIVERSITY OF MASSACHUSETTS
ARRA-DNA Mismatch and Double-Strand Break Repair Homologous recombination is a mechanism that is essential to allow cells to tolerate DNA damage produced by various DNA damaging agents. The long term goals of the parent proposal are to define the mechanisms by which agents such as cisplatin, nitric oxide and methylators induce the formation of DNA double-strand breaks. The mechanisms by which homologous recombination repairs such breaks will also be defined. Three specific aims were proposed in the parent grant to achieve the long-term goals. The second aim seeks to determine the gene products necessary for replication fork reversal which has been postulated to occur when a fork encounters a DNA drug adduct. Fork reversal is followed by homologous recombination. It is this aim that is the focus of this supplement.