UNIVERSITY OF ARKANSAS SYSTEM
The supplement request is a direct continuation of the parent grant. It follows the same idea and strategy, but deepens the approach by studying trafficking of endonucleases by using state-of-the-art equipment. The hypothesis of the overall project is that (a) during cisplatin kidney injury, premortem enzymatic DNA damage is induced by endonuclease EndoG which acts along the pathway initiated by another endonuclease, DNase I, and (b) the inactivation of EndoG may protect the kidney against injury induced by cisplatin. The supplement will accelerate the trafficking studies of endonucleases in tubular epithelial cell injury induced by cisplatin. It will allow purchase of an XYZ stage with CO2/temperature/humidity controller (Hitschfel Instruments) necessary for the proposed studies. In addition, the funds will allow continuation of the employment of a Research Technologist.