UNIVERSITY OF FLORIDA
The main objective of the parent grantis to determine the host specific factors that confer susceptibility to urinary tract infection (UTI) complicated by struvite baldder stone foramtion. The parent grant uses a rodent model of UTI caused by the human pathogen Ureaplasma parvum. As part of the studies performed within the parent grant, it was found that aberrant expression of the pro-inflammatory biomarker, S100A8 and transient infiltration of bladder tissue by eosinophils are hallmarks of complicated disease. These elements can be important in promoting a pro-inflammatory feedback loop that generates the chronic active inflammation that occurs in complicated UTI. However, the presence of both of these factors during the inflammatory response can also be influenced by the manner in which U. parvum is colonizing host tissues. Therefore, in order to adequately address the main objective of the parent grant, it will be necessary to characterize the behavior of U. parvum that is associated with each clinical profile of UTI. The goal of this research supplement is to elucidate the microbe specific factors associated with complicated UTI. The goals of this supplement will be met through the following specific aims: 1) correlate location of U. parvum in urinary tract tissues to inflammatory response, and 2) identify U. parvum proteins associated with complicated infection.