MASSACHUSETTS GENERAL HOSPITAL, THE
Our overall goal is to better understand the growth and proliferation of white adipose tissue at a cellular level so that we might learn to alter the enlargement of this tissue and provide an effective therapy for obesity. Our main hypothesis is that the proliferation of mature fat cells drives adipose tissue growth and contributes to the development of obesity. To better understand the turnover, replication, maintenance and expansion of adipocytes and the role these cellular processes play in obesity we propose the following specific aims: Aim I. Characterize replication and turnover of adipocytes in normal and modified adipose tissue. Aim II. Genetic manipulation of adipocytes. Aim III. Examine the role of genes linked to lipomatous diseases in adipocyte replication. Ultimately, the goal of these experiments is to 1) measure the adipocyte turnover in adipose tissues, 2) determine the origin of new adipocytes in adipose tissue, 3) examine the cellular processes involved in response to a loss of adipocytes in adipose tissue, 4) understand the consequence of blocking replication of adipocytes in terms of adipose tissue maintenance and growth, and 5) identify key molecules involved in adipocyte replication and to exploit these as possible points of intervention for the treatment of obesity.
| AWARD OVERVIEW |
| Award Number |
1R56DK088895-01 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$238,550 |
Project Location - City |
Boston |
| Award Date |
07/05/2010 |
Project Location - State |
MA |
| Project Status |
Completed |
Project Location - Zip |
02114-0000
|
| Jobs Reported |
0.00 |
Congressional District |
09 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
MASSACHUSETTS GENERAL HOSPITAL, THE |
| Recipient DUNS Number |
073130411
|
| Recipient Address |
55 FRUIT ST |
| Recipient City |
BOSTON |
| Recipient State |
Massachusetts |
| Recipient Zip |
02114-2621 |
| Recipient Congressional District |
09 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
A study of the turnover, maintenance and expansion of adipocytes in adult mice |
| Project Status |
Completed |
| Final Project Report Submitted |
Yes |
| Project Activities Description |
General Medical and Surgical Hospitals |
| Quarterly Activities/Project Description |
Our main goal is to understand what drives adipose tissue growth and to determine if these processes contribute to the development of obesity. To better understand the turnover, maintenance and expansion of adipocytes and the role these cellular processes play in obesity we are conducting several experiments designed to identify the origin of new adipocytes in adult adipose tissue, the molecular pathways involved in adipocyte turnover as well as the ways in which it is dysfunctional in diseases such as diabetes. To achieve this goal we have proposed three specific aims and are currently working on Specific Aim II.
Aim II. Genetic manipulation of adipocytes. We plan to determine the origin of new adipocytes in the adipose tissue of mice by performing genetic lineage analysis. We will also genetically ablate adipocytes in the adipose tissue of adult mice. Lastly, we will use transgenic mice to manipulate the cell cycle of adipocytes in the adipose tissue of mice.
1. Perform genetic lineage analysis of adipocytes.
2. Generate transgenic mice that carry the Leptin promoter driving the Cre recombinase fused with the Estrogen Receptor.
3. Genetically ablate adipocytes in adult mice.
Taken together these data will allow us to determine 1) the origin of new adipocytes in adipose tissue and 2) the cellular processes involved in response to a loss of adipocytes in adipose tissue
|
| Jobs Created |
0.00 |
| Description of Jobs Created |
This mechanism allowed for the retainment of a talented rotation research fellow, to continue his research.
The aim of our study is to understand the formation, maintenance, and function of fat cells and to gain a greater understanding of obesity and obesity related diseases. Our main challenge is to understand the origin and regulation of cells that give rise to fat cells and to characterize the genetic and molecular mechanisms that specify fat cell fates. As a result of Recovery Act funded work, we were able to provide a full year of funding to a Massachusetts General Hospital surgical fellow Frank Lau MD, who is currently working full-time on several the projects described in our original R01 submission to the NIH. In addition, we have been able to increase the size of our mouse colony and have employed a research technician out of start-up funds to work directly on these projects.
|
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0883 |
| Award Information |
| Award Date |
07/05/2010 |
| Award Number |
1R56DK088895-01 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$238,550 |
| Funds Invoiced/Received |
$238,550 |
| Expenditure Amount |
$238,550 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
622110 |
| Activity Description |
General Medical and Surgical Hospitals |
| Sub-Awards Information |
| Sub-awards to Organizations |
0 |
| Sub-award Amounts to Organizations |
$0 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
0 |
| Total Amount of payments to vendors greater than $25,000/award |
$0 |
| Number of payments to vendors less than $25,000/award |
121 |
| Total Amount of payments to vendors less than $25,000/award |
$34,664 |
| Location Information |
| Latitude, Longitude |
42º 21' 44",
-71º 4' 11" |
| Congressional District |
09 |
| Address 1 |
55 Fruit Street |
| Address 2 |
|
| City |
Boston |
| County |
Suffolk |
| State |
MA |
| Zip |
02114-0000 |
|
 |