Award will support the purchase of a set of plant growth chambers that can be set for different temperatures, humidity, photoperiods, and CO2 levels. Aquisition of this instrumentation will have a direct impact on the research of three faculty and will aid the research programs of several others. Chambers will also see extensive use in several courses and in undergraduate research projects. Specifically, one chamber will be set at low temperature (10 C), one chamber will be set at 25 C and long days (16 L:8D), one chamber will be set at 25 C and short days (8L:16D), one chamber set at high temperature (40C), one chamber that will be set at 25 C and elevated CO2 and a final “quarantine” chamber may be used to evaluate effects of pathogens, insects or environmental toxins. Dr. Terzaghi, in collaboration with Dr. Xing Wang Deng at Yale University, is screening Arabidopsis mutants with DNA insertions in non-coding RNAs recently identified by high-throughput sequencing. The ability to screen for altered responses to temperature, photoperiod, CO2 or other factors in parallel rather than in series will greatly speed his research. Dr. Klemow, in collaboration with Drs. Steele and Terzaghi, studies both rare native and invasive plant species, as well as revegetation of abandoned minelands, so the ability to evaluate the effects of altered temperature and other environmental factors on these species will greatly enhance his program. Dr. Steele studies the regeneration of native forests, in collaboration with Drs. Klemow and Terzaghi and Dr. Robert Swihart of Purdue University. The ability to study the effects of temperature and other environmental factors on germination and on establishment and physiology of native species such as chestnuts and various oaks will greatly aid his research. In addition, Dr. Biggers studies the reproduction of endangered native mussels, and the ability to control temperature and photoperiod will aid his program as well. The ability to compare the growth of plants under a variety of conditions will also enhance several courses, including the second semester of introductory biology and upper-levels including plant physiology, plant form and function, and ecology. It will also enhance the opportunities for independent and senior research projects.