HARVARD COLLEGE, PRESIDENT & FELLOWS OF
Chronic metabolic diseases, such as obesity, insulin resistance, type 2 diabetes, and cardiovascular disease are among the most common diseases with adverse effects on global health. Despite their enormous burden on human life, the preventive and therapeutic opportunities are limited and there is ongoing need for new and more effective remedies. Our project aims to identify core mechanisms that give rise to these pathologies by focusing on the emerging and exciting concept of organelle dysfunction in metabolic diseases. In this proposal we will study the causes of organelle failure focusing on endoplasmic reticulum and using systematic high-throughput approaches to identify all regulated lipids and proteins in purified organelles from control and disease-afflicted tissues. The mechanism uncovered through these experiments will be instrumental in the design and implementation of preventive and therapeutic strategies and allow the general field reagents and technologies to explore the biological effects and mechanisms of nutrient exposures. The proposed studies are in excellent match with the several themes of the initiative.
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| AWARD OVERVIEW |
| Award Number |
1RC4DK090942-01 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$2,363,388 |
Project Location - City |
Boston |
| Award Date |
09/30/2010 |
Project Location - State |
MA |
| Project Status |
More than 50% Completed |
Project Location - Zip |
02115-6096
|
| Jobs Reported |
3.09 |
Congressional District |
07 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
HARVARD COLLEGE, PRESIDENT & FELLOWS OF |
| Recipient DUNS Number |
149617367
|
| Recipient Address |
677 HUNTINGTON AVE |
| Recipient City |
BOSTON |
| Recipient State |
Massachusetts |
| Recipient Zip |
02115-6028 |
| Recipient Congressional District |
07 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
Deconvolution of metabolic adaptation of endoplasmic reticulum |
| Project Status |
More than 50% Completed |
| Final Project Report Submitted |
No |
| Project Activities Description |
Colleges, Universities, and Professional Schools |
| Quarterly Activities/Project Description |
Our recent work has been focused on pathways of inflammation and hypoxia contributing to endoplasmic reticulum (ER) stress in adipose tissue. We performed anti-inflammation treatment of the obese mice and examined markers of ER stress in the adipose tissue. We also examined the induction of oxidative stress as well as hypoxia in differentiated 3T3-L1 cells and markers of ER stress. We performed co-culture experiments of macrophage and differentiated 3T3-L1 cells to study the connection between macrophage infiltration and ER stress. We will also study macrophage chemotaxis in the co-culture conditions to examine the role of adipocyte ER stress in macrophage recruitment. Over the past several months, we have been back crossing p53 knock-out mice to C57 background. Once we obtain genome scale homogeneity, we will use the p53KO mice to examine the connection between DNA damage, inflammation and ER stress in the obese adipose tissues. Through this work, we hope to establish the mechanistic connection between hypoxia, inflammation and ER stress in the obese adipose tissues. |
| Jobs Created |
3.09 |
| Description of Jobs Created |
Research Assistant; Research Associate; Research Associate; Research Associate; Postdoctoral Research Fellow; Laboratory Safety and Compliance Manager |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0883 |
| Award Information |
| Award Date |
09/30/2010 |
| Award Number |
1RC4DK090942-01 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$2,363,388 |
| Funds Invoiced/Received |
$1,880,079 |
| Expenditure Amount |
$1,921,877 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
611310 |
| Activity Description |
Colleges, Universities, and Professional Schools |
| Sub-Awards Information |
| Sub-awards to Organizations |
0 |
| Sub-award Amounts to Organizations |
$0 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
1 |
| Total Amount of payments to vendors greater than $25,000/award |
$38,892 |
| Number of payments to vendors less than $25,000/award |
1462 |
| Total Amount of payments to vendors less than $25,000/award |
$352,166 |
Partners Healthcare System, Inc - Award Number 1RC4DK090942-01 - Partners Healthcare System, Inc
| Award Number |
1RC4DK090942-01 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
825636988 |
| Vendor HQ Zip Code + 4 |
02199-8123 |
| Vendor Name |
Partners Healthcare System, Inc |
| Product and Service Description |
SERVICES |
| Payment Amount |
$38,892 |
| Location Information |
| Latitude, Longitude |
42º 20' 7",
-71º 6' 7" |
| Congressional District |
07 |
| Address 1 |
|
| Address 2 |
|
| City |
Boston |
| County |
Suffolk |
| State |
MA |
| Zip |
02115-6096 |
|
 |