UNIVERSITY OF PUERTO RICO-MEDICAL SCIENCES CAMPUS
This proposal will focus on HIV infection studies with HIV-ADA and will not compare different HIV isolates. Primary isolates will be performed in future studies. The scope is limited to cystatins B/C and cathepsin B. Includes studies of MDM and supernatants for the expression and secretion of cystatins B/C, cathepsin B, and neurotoxic potential from HAART patients to include mechanisms.
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| AWARD OVERVIEW |
| Award Number |
1R01MH083516-01 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$1,000,452 |
Project Location - City |
San Juan |
| Award Date |
06/19/2009 |
Project Location - State |
PR |
| Project Status |
Completed |
Project Location - Zip |
00936-5067
|
| Jobs Reported |
0.80 |
Congressional District |
98 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
UNIVERSITY OF PUERTO RICO-MEDICAL SCIENCES CAMPUS |
| Recipient DUNS Number |
948108063
|
| Recipient Address |
PUERTO RICO MEDICAL CENTER |
| Recipient City |
SAN JUAN |
| Recipient State |
Puerto Rico |
| Recipient Zip |
00935-5067 |
| Recipient Congressional District |
98 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
Modulation of Cystatins and Cathepsins in HIV-1 Neuropathogenesis |
| Project Status |
Completed |
| Final Project Report Submitted |
Yes |
| Project Activities Description |
HIV/AIDS Research |
| Quarterly Activities/Project Description |
Our laboratory has shown increased secretion and neurotoxicity of cathepsin B from in vitro HIV-infected monocyte-derived macrophages, and increased expression in post-mortem brain tissue with HIV encephalitis and HAD. We hypothesized that cystatin B and cathepsin B could represent potential biomarkers for disease progression. Monocytes, plasma, and CSF from 98 HIV seropositive Hispanic women cohort were selected for this study. These included 75 patient visits stratified by normal (22), asymptomatic neurocognitive impairment (ANI) (23), and HAD (28). Thirty-two patients at two consecutive visits were stratified into progressors and non-progressors. Samples were evaluated for cystatins B and C, and cathepsin B expression and activity. Data was analyzed using Kruskal-Wallis one-way analysis of variance. Increased cathepsin B and cystatins B and C were found in plasma of HIV seropositive women. Higher expression of cathepsin B and cystatin B were found in monocytes from women with HAD (p<0.05). Significant decrease in cathepsin B activity in CSF was found in demented compared to asymptomatic women and in monocytes from progressors. These results demonstrate that dysregulation of cystatin B-cathepsin B system is operative in HIV associated neurocognitive disorders (HAND) and suggest that expression of cystatin B and cathepsin B in monocytes could be potential biomarker for disease progression.
Overall Significance of this project. Our results are important for diagnosis and therapy of HAND and have been published in the J Neurovirology (Rivera et al, 2011), PlosONE (Rodriguez-Franco et al, 2012), and the patient-related research will be submitted to AIDS.
|
| Jobs Created |
0.80 |
| Description of Jobs Created |
One PI (1.2cal);Two Collaborators (1.0 cal); Ono Research Assistant (6.0cal/mo); one project administrator (0.5 cal); . This project benefits three well trained personnel and the traininig of a PHD candidate. |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0907 |
| Award Information |
| Award Date |
06/19/2009 |
| Award Number |
1R01MH083516-01 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$1,000,452 |
| Funds Invoiced/Received |
$1,000,452 |
| Expenditure Amount |
$1,000,452 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
H02.13.03 |
| Activity Description |
HIV/AIDS Research |
| Sub-Awards Information |
| Sub-awards to Organizations |
1 |
| Sub-award Amounts to Organizations |
$134,422 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
0 |
| Total Amount of payments to vendors greater than $25,000/award |
$0 |
| Number of payments to vendors less than $25,000/award |
193 |
| Total Amount of payments to vendors less than $25,000/award |
$134,546 |
Sub-award 2010-000694 - UNIVERSITY OF NEBRASKA
| Sub-Award Amount |
$134,422 |
| Sub-Award Date |
09/29/2009 |
| Sub-Awards Disbursed |
$104,680.19 |
| Project Location - City |
Omaha |
| Project Location - State |
NE |
| Project Location - Zip Code |
68198-7835 |
| Project Location - Congressional District |
02 |
| Sub-Recipient DUNS Number |
168559177
|
| Sub-Recipient Address |
987835 NEBRASKA MEDICAL CENTER |
| Sub-Recipient City |
OMAHA |
| Sub-Recipient State |
Nebraska |
| Sub-Recipient Zip Code |
68198-6810 |
| Sub-Recipient Congressional District |
02 |
Required To Report Top 5
Highly Compensated Officials |
No |
| Location Information |
| Latitude, Longitude |
18º 25' 3",
-66º 4' 34" |
| Congressional District |
98 |
| Address 1 |
University of Puerto Rico Medical Sciences Campus |
| Address 2 |
Biomedical Bldg Rm 301 |
| City |
San Juan |
| County |
San Juan |
| State |
PR |
| Zip |
00936-5067 |
|
 |