Grants - AWARD SUMMARY


UNIVERSITY OF MASSACHUSETTS


We will conduct two studies that will serve to improve and enable the accomplishment of the specific aims of 5R01 GM030758: 28-29. The first addresses time and data loss due to human cells migrating off the fields of view during the time lapse observations used for all specific aims. We will make and post on the web a series of 20-63X phase contrast and differential interference contrast time lapse movies of normal human cells during interphase and mitosis. These movies will be used to adapt an existing fluorescent cell tracking algorithm for transmitted light applications. This will allow a motorized microscope stage to keep individual human cells centered in the field of view as they migrate. The movies will also be available to computer scientists to be used in developing algorithms for pattern recognition and analysis. The second project addresses the phototoxicity of the 488nm light used to excite the GFP in many applications with live cells. We will test reports that short (a few milliseconds) but intense bursts of blue light are less damaging to the cells than longer but less intense exposures.

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AWARD OVERVIEW

AWARD OVERVIEW
Award Number 3R01GM030758-28S2 Funding Agency Department of Health and Human Services
Total Award Amount $186,599 Project Location - City Worcester
Award Date 09/30/2009 Project Location - State MA
Project Status Completed Project Location - Zip 01655-0002
Jobs Reported 1.00 Congressional District 03
Project Location - Country US

Recipient Information (Grants)

Recipient Information (Grants)
Recipient Name UNIVERSITY OF MASSACHUSETTS
Recipient DUNS Number 603847393
Recipient Address 55 LAKE AVE N
Recipient City WORCESTER
Recipient State Massachusetts
Recipient Zip 01655-0002
Recipient Congressional District 03
Recipient Country USA
Required to Report Top 5
Highly Compensated Officials		 No

Projects and Jobs Information

Projects and Jobs Information
Project Title Centrosome reduplication and consequences of cleavage failure/prolonged mitosis
Project Status Completed
Final Project Report Submitted Yes
Project Activities Description Cancer Research
Quarterly Activities/Project Description "In the past quarter we uploaded 11 films to the American Society for Cell Biology (ASCB) image archive called ""The Cell, an Image Library"". These submissions are each held in two formats: movie and image stacks; the image stacks are provided so that users can step through the sequences an image at a time in a planned and controlled way. The same films in movie format can be used for faster viewing and better analysis of cell dynamics. The submissions are scheduled to go into the ""sofware training"" category in the archive. These films are of untransformed human cells during interphase and mitosis. For the interphase sequences we provided films with different cell densities and at different magnifications. Some of the films are of cells arrested in G1 so as to not present users with the complications of cells dividing and increasing in number. The films of dividing cells are all taken at high magnification and cover the entire range of mitotic processes. The purpose of all these films is to provide imaging sofware engineers with image sequences for development of programs for pattern recognition,object segmentation ,cell tracking, and a baseline image set for normal morphology and dynamics against which to compare drug treated cells that might show subtle differences. In providing these films, we helped the ASCB staff work out problems and unforseen issues with their acquisition and archiving routines. Multiple submissions were required along with work on file formats. The movies and their corresponding image stacks have been archived. The final invoicing for the project will be complete in the next couple weeks."
Jobs Created 1.00
Description of Jobs Created Post Doc Assoc


Purchaser Information (Grants)

Purchaser Information
Contracting Office ID Not Reported
Contracting Office Name Not Available
Contracting Office Region Not Available
TAS Major Program 75-0852

Award Information

Award Information
Award Date 09/30/2009
Award Number 3R01GM030758-28S2
Order Number
Award Type Grants
Funding Agency ID 75
Funding Agency Name Department of Health and Human Services
Funding Office Name Not Available
Awarding Agency ID 75
Awarding Agency Name Department of Health and Human Services
Amount of Award $186,599
Funds Invoiced/Received $183,316
Expenditure Amount $183,316
Infrastructure Expenditure Amount $0
Infrastructure Purpose and Rationale Not Reported
Infrastructure Point of Contact Name Not Reported
Infrastructure Point of Contact Email Not Reported
Infrastructure Point of Contact Phone Not Reported
Infrastructure Point of Contact Address Not Reported
Infrastructure Point of Contact City Not Reported
Infrastructure Point of Contact State Not Reported
Infrastructure Point of Contact Zip Not Reported

Product or Service Information (Grants)

Product or Service Information
Primary Activity Code H02.04
Activity Description Cancer Research

Sub-Awards Information

Sub-Awards Information
Sub-awards to Organizations 0
Sub-award Amounts to Organizations $0
Sub-Awards to Individuals 0
Sub-Award Amounts to Individuals $0
Number of Sub-awards less than $25,000/award 0
Amount of Sub-awards less than $25,000/award $0
Number of payments to vendors greater than $25,000 0
Total Amount of payments to vendors greater than $25,000/award $0
Number of payments to vendors less than $25,000/award 72
Total Amount of payments to vendors less than $25,000/award $12,891







Project Location Detail

Location Information
Latitude, Longitude 42º 16' 39", -71º 45' 33"
Congressional District 03
Address 1 55 Lake Avenue North
Address 2
City Worcester
County Worcester
State MA
Zip 01655-0002
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