Recent progress in podocyte biology suggests that glomerular capsular cell, namely glomerular parietal cells have the potential to contribute to podocyte regeneration/renal repair after kidney development concludes. My results derived from projects supported by the parent K01 grant also suggested that glomeurli may regain podocytes gradually after low level podocyte loss; transgenic expression of telomerase reverse transcriptase (TERT) in podocytes induced podocyte proliferation. Based on these data, this supplement proposal hypothesized that, in vitro expanded podocytes are excellent source for cell replacement therapy. We will establish and characterize a new inducible podocyte cell line based on the inducible, podocyte specificTERT transgenic mice and test its therapeutic potential by a co-culture system in vitro and transplantation in vivo. This is a valuable supplement to the existing parent grant within the same scope of comparing the efficiency and capacity of different sources of (stem) cells in renal repair. The proposed supplemental project will also provide the research community a new research tool (conditionally immortal podocyte cell line) which can be maintained more cost-effectively. More importantly, with the support of this administrative supplement, a current part-time research assistant will have her weekly hours extended. Moreover, the supplement will contribute to the survival and well-beings of the industrial chain downstream of biomedical research institutions.