COLD SPRING HARBOR LABORATORY ASSOCIATION, INC.
We are requesting funding for the creation of a next generation sequencing center, dedicated to plant genomics. Such centers were recently called for by a report on the plant genome project by the National Research Council. We will use the facility for a wide range of projects ranging from de novo sequencing of the various plant genomes and transcriptomes to studies of epigenetics. The proposed center will consist of two Illumina sequencers and computer equipment for data analysis and storage and a dedicated technician to operate the instruments. We have already committed considerable time and effort to this technology. The requested funds would allow us to contribute the experience gained to create a state of the art sequencing center for plant genomics. Such centers exist of course for human and animal sequencing, but dedicated instruments for plant sequencing will be an important development that would greatly impact plant genomics. The availability of substantial capability in next generation sequencing will allow the much more rapid collection of plant genomics data. Genomics is at an inflection point due to incredible changes in technology. We have been an early user of this technology, and have built a center capable of generating as much as 30 billion bases of high quality sequence per week. We are requesting funds to provide equipment to allow us to use that knowledge to leverage the productivity of a center specifically for plant genomics. The impact of such a center on the field of plant genomics should be significant. We have already provided capacity of this type to several groups outside our institution, as well as within, and allowing us to dedicate capacity to plant genomics would allow us to expand this effort. The proposed center would greatly enhance the national infrastructure for next generation sequencing, that is available to the plant genome community, as well as the training of scientists to use such equipment.