UNIVERSITY OF MASSACHUSETTS
This program will continue to study fundamental structural properties of the cell nucleus that support biological control and are disrupted in leukemia and metastatic breast cancer. We will gain insight into components of nuclear organization that can be targeted for innovative cancer therapies.This Administrative Supplement for the parent P01 Nuclear Structure and Gene Expression? requests funds to expedite and enhance key collaborative objectives among Program Project investigators. We continue to pursue our hypothesis that parameters of nuclear architecture support cell growth and phenotypic properties of normal and tumor cells by facilitating the organization of chromosomes, genes, transcripts and regulatory complexes as dynamic three-dimensional micro-environments within the nucleus. We propose the following Supplemental Approaches to continue to characterize functional relationships between nuclear organization and fidelity of gene regulation: Supplemental Approach 1: Characterization of chromatin-mediated mechanisms supporting tumorigenesis-related gene regulation at subnuclear domains. We will perform high resolution chromatin mapping of Runx controlled genes and Runx dependent post-translational modifications of histone proteins that require fidelity of subnuclear targeting in leukemia and breast cancer cells, as well as during three-dimensional breast epithelial tissue development in culture (collaboration between Projects 1, 2 and 4). Supplemental Approach 2: Examination of spatio-temporal and epigenetic parameters of subnuclear targeting and chromosomal association of Runx proteins during interphase and mitosis. We will apply high resolution immunofluorescence microscopy including time-lapse analysis of live cells and biochemical analyses to define temporal-spatial and functional relationships between Runx and Brg1 proteins and selected Runx-related co-factors. We will also define epigenetic states of Runx foci during interphase and mitosis in normal and cancer cells (collaboration between Projects 1, 2 and 4). This Supplement will enhance our capabilities to execute the original Specific Aims of the P01 Program and will expedite the discovery of epigenetic mechanisms linked to subnuclear targeting that are deregulated in cancer.
Choose a quarter and click "Go."
| AWARD OVERVIEW |
| Award Number |
3P01CA082834-09S1 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$669,529 |
Project Location - City |
Worcester |
| Award Date |
09/02/2009 |
Project Location - State |
MA |
| Project Status |
Completed |
Project Location - Zip |
01655-0002
|
| Jobs Reported |
2.07 |
Congressional District |
03 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
UNIVERSITY OF MASSACHUSETTS |
| Recipient DUNS Number |
603847393
|
| Recipient Address |
55 LAKE AVE N |
| Recipient City |
WORCESTER |
| Recipient State |
Massachusetts |
| Recipient Zip |
01655-0002 |
| Recipient Congressional District |
03 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
Nuclear Structure and Gene Expression |
| Project Status |
Completed |
| Final Project Report Submitted |
Yes |
| Project Activities Description |
Medical Research, General/Other |
| Quarterly Activities/Project Description |
High-resolution chromatin analysis (ChIP-DeepSeq) of leukemic cells has been finalized and a manuscript describing these results is near submission. ChIP-DeepSeq analyses of breast cancer cells using multiple antibodies, as well as Brg1-mediated regulation of growth of breast cancer cells, will continue as part of the parent P01 grant. Immunofluorescence analyses of co-regulatory proteins will be performed as necessary to support other studies in the parent P01. The final expenses will be invoiced in the next couple weeks |
| Jobs Created |
2.07 |
| Description of Jobs Created |
Post Doc Assoc, 2 Research Associates, Research Lab Tech |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0850 |
| Award Information |
| Award Date |
09/02/2009 |
| Award Number |
3P01CA082834-09S1 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$669,529 |
| Funds Invoiced/Received |
$669,525 |
| Expenditure Amount |
$669,529 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
H01 |
| Activity Description |
Medical Research, General/Other |
| Sub-Awards Information |
| Sub-awards to Organizations |
0 |
| Sub-award Amounts to Organizations |
$0 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
1 |
| Total Amount of payments to vendors greater than $25,000/award |
$34,885 |
| Number of payments to vendors less than $25,000/award |
238 |
| Total Amount of payments to vendors less than $25,000/award |
$125,020 |
APPLIED BIOSYSTEMS, LLC - Award Number 3P01CA082834-09S1 - APPLIED BIOSYSTEMS, LLC
| Award Number |
3P01CA082834-09S1 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
001184480 |
| Vendor HQ Zip Code + 4 |
94404-1128 |
| Vendor Name |
APPLIED BIOSYSTEMS, LLC |
| Product and Service Description |
Research/Educational Equipment |
| Payment Amount |
$34,885 |
| Location Information |
| Latitude, Longitude |
42º 16' 39",
-71º 45' 33" |
| Congressional District |
03 |
| Address 1 |
55 Lake Avenue North |
| Address 2 |
|
| City |
Worcester |
| County |
Worcester |
| State |
MA |
| Zip |
01655-0002 |
|
 |