UNIVERSITY OF MASSACHUSETTS
Assembly and disassembly of proteins, natures macromolecules, in response to a specific stimulus is ubiquitous in biology. Mimicking these processes, where proteins are used as the stimulus to disassemble artificial macromolecular assemblies are interesting for a variety of biomedical applications. For example, although targeted drug delivery is not a specific goal for this proposal, the experiments proposed here to obtain the proof-of-concept could have a significant impact in this area. Specifically, this proposal describes the development of versatile strategies to disassemble dendrimer-based amphiphilic assemblies using proteins as stimuli. Facially amphiphilic dendrimers, reported by us recently in the literature, are a unique class of molecules in that these are the only class of amphiphilic dendrimers that form micelle-type assemblies through aggregation. Even more interesting is the fact that these assemblies can be disassembled in response to a stimulus. While stimuli-responsive assemblies themselves have been studied quite extensively, reports where proteins are used as the stimulus for disassembling organized supramolecular assemblies are rather limited. This project describes a concerted approach to this problem, which could have broad implications in biology and medicine.
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| AWARD OVERVIEW |
| Award Number |
2R01GM065255-07A1 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$536,260 |
Project Location - City |
AMHERST |
| Award Date |
08/31/2009 |
Project Location - State |
MA |
| Project Status |
Completed |
Project Location - Zip |
01003-9242
|
| Jobs Reported |
0.00 |
Congressional District |
01 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
UNIVERSITY OF MASSACHUSETTS |
| Recipient DUNS Number |
153926712
|
| Recipient Address |
70 BUTTERFIELD TERRACE |
| Recipient City |
AMHERST |
| Recipient State |
Massachusetts |
| Recipient Zip |
01003-9242 |
| Recipient Congressional District |
01 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
New Amphiphilic Dendrimers for Encapsulation and Release |
| Project Status |
Completed |
| Final Project Report Submitted |
Yes |
| Project Activities Description |
Human Services, General/Other |
| Quarterly Activities/Project Description |
During this period, we have developed a ligand functionalized dendrimer that buries its hydrophobic moiety within the lipophililic interior of the micelle-type assembly. When this assembly is exposed to the complementary protein (in this case, anti-2,4-DNP-IgG), we inquired whether the ligand will be available for binding-induced disassembly. Our results show that these dendrimer-based assemblies are indeed available for binding-induced disassembly. This disassembly is possible, only if the mechanistic pathway that takes advantage of the equilibrium between the monomeric state of the dendron and the aggregate state is available for the binding-induced disassembly. This finding significantly expands the repertoire of binding-induced disassembly for specific protein-based detection and therapeutic technologies. There will be no additional expenditures or amounts to be drawn for this award. |
| Jobs Created |
0.00 |
| Description of Jobs Created |
No jobs were created or retained |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0852 |
| Award Information |
| Award Date |
08/31/2009 |
| Award Number |
2R01GM065255-07A1 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$536,260 |
| Funds Invoiced/Received |
$535,475 |
| Expenditure Amount |
$535,475 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
P01 |
| Activity Description |
Human Services, General/Other |
| Sub-Awards Information |
| Sub-awards to Organizations |
0 |
| Sub-award Amounts to Organizations |
$0 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
1 |
| Total Amount of payments to vendors greater than $25,000/award |
$43,436 |
| Number of payments to vendors less than $25,000/award |
242 |
| Total Amount of payments to vendors less than $25,000/award |
$43,843 |
Teledyne Isco, Inc. - Award Number 2R01GM065255-07A1 - Teledyne Isco, Inc.
| Award Number |
2R01GM065255-07A1 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
007274194 |
| Vendor HQ Zip Code + 4 |
68504-1328 |
| Vendor Name |
Teledyne Isco, Inc. |
| Product and Service Description |
Freight |
| Payment Amount |
$43,436 |
| Location Information |
| Latitude, Longitude |
42º 23' 28",
-72º 31' 27" |
| Congressional District |
01 |
| Address 1 |
70 BUTTERFIELD TERRACE |
| Address 2 |
OFFICE OF GRANTS AND CONTRACTS |
| City |
AMHERST |
| County |
Hampshire |
| State |
MA |
| Zip |
01003-9242 |
|
 |