UNIVERSITY OF WISCONSIN SYSTEM
DESCRIPTION (provided by applicant): Methylmercury (MeHg) is a ubiquitous environmental pollutant. The neurotoxicity of MeHg and the high susceptibility of the developing brain are well established both in humans and experimental animals. Prenatally exposed children display a range of effects varying from severe cerebral palsy to subtle developmental delays. Many of the more subtle effects of developmental MeHg exposure can be permanent, including behavioral abnormalities and loss of IQ, and impart a significant cost to society as a whole. It is estimated that as many as 15% of American women of childbearing age have total blood mercury concentrations at a level above that associated with loss of IQ (> 3.5 3.5
Choose a quarter and click "Go."
| AWARD OVERVIEW |
| Award Number |
3R21ES01910401S1 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$878,148 |
Project Location - City |
Milwaukee |
| Award Date |
09/30/2009 |
Project Location - State |
WI |
| Project Status |
More than 50% Completed |
Project Location - Zip |
53201-0340
|
| Jobs Reported |
0.00 |
Congressional District |
04 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
UNIVERSITY OF WISCONSIN SYSTEM |
| Recipient DUNS Number |
627906399
|
| Recipient Address |
3203 N DOWNER AVE STE 73 |
| Recipient City |
MILWAUKEE |
| Recipient State |
Wisconsin |
| Recipient Zip |
53211-3153 |
| Recipient Congressional District |
04 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
Influence of human gene variants on the effects of developmental MeHg exposure |
| Project Status |
More than 50% Completed |
| Final Project Report Submitted |
No |
| Project Activities Description |
Medical Research, General/Other |
| Quarterly Activities/Project Description |
We have designed both the zinc finger nucleases and transgenesis constructs. Zinc finger nuclease constructs are in hand and are being injected to create the knockout animals. Based on the technical challenges we have faced with this technique, we have also been working on a back-up plan using TALENs to generate knockout animals. For transgenesis, founders have been identified for at least three lines for each transgene. While the animals are in quarantine, we continue to perform outcrosses to ensure single insertions and in-breedings to determine if insertions sites interfere with critical genes. Based on this analysis we expect one of our knockouts to be embryonic lethal. The Graduate Research Assistant and Postdoctoral Research Associate have been working with gene knockdown technology and are prepared to phenotype the knockouts. Through our knockdown studies, we only expect one knockout to be embryonic lethal. However, we now know that the human gene can complement the knockout and fish will survive. When we phenocopy the human polymorphisms (e.g. reduce the functional protein expression level), the embryonic zebrafish are healthy under normal husbandry conditions. We have learned the new analytical techniques that will be used to analyze the fish lines carrying the human genes. We have positive control ZFNs to use and are working on the techniques to detect strand breaks. Work is underway on the analysis of GSH levels in tissues of normal and knockdown animals. |
| Jobs Created |
0.00 |
| Description of Jobs Created |
None. |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0863 |
| Award Information |
| Award Date |
09/30/2009 |
| Award Number |
3R21ES01910401S1 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$878,148 |
| Funds Invoiced/Received |
$878,148 |
| Expenditure Amount |
$875,691 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
H01 |
| Activity Description |
Medical Research, General/Other |
| Sub-Awards Information |
| Sub-awards to Organizations |
3 |
| Sub-award Amounts to Organizations |
$165,441 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
3 |
| Total Amount of payments to vendors greater than $25,000/award |
$75,023 |
| Number of payments to vendors less than $25,000/award |
489 |
| Total Amount of payments to vendors less than $25,000/award |
$124,963 |
Sub-award 103405506 - AMERICAN UNIVERSITY
| Sub-Award Amount |
$33,445 |
| Sub-Award Date |
12/10/2009 |
| Sub-Awards Disbursed |
$33,418.56 |
| Project Location - City |
Washington |
| Project Location - State |
DC |
| Project Location - Zip Code |
20016-8001 |
| Project Location - Congressional District |
01 |
| Sub-Recipient DUNS Number |
077795060
|
| Sub-Recipient Address |
4400 MASSACHUSETTS AVE, NW |
| Sub-Recipient City |
WASHINGTON |
| Sub-Recipient State |
District of Columbia |
| Sub-Recipient Zip Code |
20016-8001 |
| Sub-Recipient Congressional District |
01 |
Required To Report Top 5
Highly Compensated Officials |
No |
Sub-award 103405505 - ANN & ROBERT H. LURIE CHILDREN'S HOSPITAL OF CHICAGO
| Sub-Award Amount |
$87,632 |
| Sub-Award Date |
10/12/2009 |
| Sub-Awards Disbursed |
$87,632.00 |
| Project Location - City |
Chicago |
| Project Location - State |
IL |
| Project Location - Zip Code |
60611-2991 |
| Project Location - Congressional District |
07 |
| Sub-Recipient DUNS Number |
074438755
|
| Sub-Recipient Address |
225 E. CHICAGO AVE |
| Sub-Recipient City |
CHICAGO |
| Sub-Recipient State |
Illinois |
| Sub-Recipient Zip Code |
60611-2991 |
| Sub-Recipient Congressional District |
07 |
Required To Report Top 5
Highly Compensated Officials |
No |
Sub-award 103405507 - UNIVERSITY OF TOLEDO, THE
| Sub-Award Amount |
$44,364 |
| Sub-Award Date |
10/14/2009 |
| Sub-Awards Disbursed |
$25,148.43 |
| Project Location - City |
Toledo |
| Project Location - State |
OH |
| Project Location - Zip Code |
43606-3390 |
| Project Location - Congressional District |
09 |
| Sub-Recipient DUNS Number |
051623734
|
| Sub-Recipient Address |
2801 W BANCROFT ST |
| Sub-Recipient City |
TOLEDO |
| Sub-Recipient State |
Ohio |
| Sub-Recipient Zip Code |
43606-3390 |
| Sub-Recipient Congressional District |
09 |
Required To Report Top 5
Highly Compensated Officials |
No |
SIGMA-ALDRICH CORPORATION - Award Number 3R21ES01910401S1 - SIGMA-ALDRICH CORPORATION
| Award Number |
3R21ES01910401S1 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
079928354 |
| Vendor HQ Zip Code + 4 |
63103-2530 |
| Vendor Name |
SIGMA-ALDRICH CORPORATION |
| Product and Service Description |
Custom Zinc Finger Nucleases |
| Payment Amount |
$25,008 |
SIGMA-ALDRICH CORPORATION - Award Number 3R21ES01910401S1 - SIGMA-ALDRICH CORPORATION
| Award Number |
3R21ES01910401S1 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
079928354 |
| Vendor HQ Zip Code + 4 |
63103-2530 |
| Vendor Name |
SIGMA-ALDRICH CORPORATION |
| Product and Service Description |
custom zinc finger nucleases |
| Payment Amount |
$25,008 |
SIGMA-ALDRICH CORPORATION - Award Number 3R21ES01910401S1 - SIGMA-ALDRICH CORPORATION
| Award Number |
3R21ES01910401S1 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
079928354 |
| Vendor HQ Zip Code + 4 |
63103-2530 |
| Vendor Name |
SIGMA-ALDRICH CORPORATION |
| Product and Service Description |
Custom Zinc Finger Nucleases |
| Payment Amount |
$25,008 |
| Location Information |
| Latitude, Longitude |
43º 2' 4",
-87º 54' 55" |
| Congressional District |
04 |
| Address 1 |
|
| Address 2 |
|
| City |
Milwaukee |
| County |
Milwaukee |
| State |
WI |
| Zip |
53201-0340 |
|
 |