TUFTS MEDICAL CENTER PARENT, INC.
Tanycytes are specialized cells that line the floor and ventrolateral walls of the third ventricle. Compelling evidence suggests they have an important role in generating thyroid hormone due to high expressionof type 2 iodothyronine deiodinase, an enzyment critical for conversion of the prohormone, T4, into the biological active thyroid hormone, T3. We have proposed, therefore, that tanycytes are involved in th regulation of the hypothalamic-pituitary-thyroid (HPT) axis and energy homoestasis. To facilitate the study of these cells in neuroendocrine regulation, we will identify tanycyte specific genes by laser capture microdissection and microarray analysis to develop transgenic mice that express Cre recombinase specifically in tanycytes.
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| AWARD OVERVIEW |
| Award Number |
1R21DK078998-01A2 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$427,588 |
Project Location - City |
Boston |
| Award Date |
05/08/2009 |
Project Location - State |
MA |
| Project Status |
Completed |
Project Location - Zip |
02111-1526
|
| Jobs Reported |
0.13 |
Congressional District |
08 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
TUFTS MEDICAL CENTER PARENT, INC. |
| Recipient DUNS Number |
079532263
|
| Recipient Address |
800 WASHINGTON ST |
| Recipient City |
BOSTON |
| Recipient State |
Massachusetts |
| Recipient Zip |
02111-1526 |
| Recipient Congressional District |
08 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
Tanycytes and Nonthyroid Illness |
| Project Status |
Completed |
| Final Project Report Submitted |
Yes |
| Project Activities Description |
Endocrine, Metabolic & Nutritional Research |
| Quarterly Activities/Project Description |
Because of concerns that our initial RNA-Seq analysis of tanycytes removed by LCM from the wall of the third ventricle may have also contained other cell types that are adjacent to or within the wall of the third ventricle, including arcuate nucleus AGRP/NPY neurons, infiltrating inflammatory cells (macrophages) and ependymal cells, we decided to repeat the RNA-Seq analysis on tanycytes using a different strategy that would yield highly purified material. As nestin is highly and selectively expressed in tanycytes in the adult animal, we obtained Nestin-GFP transgenic mice and purified the tanycyte population by FACS. After several attempts, we developed an approach that allowed extraction of high quality material for RNA-Seq analysis. This analysis is currently being conducted and should be available within the next two week. The resulting transcriptome will be compared to our initial data set to determine whether tanycytes can be characterized as multipotent cells with properties of inflammatory cells, stem cells and hormonal cells, as indicated in the initial RNA-Seq analysis. We are also in the process of verifying that Rax is selectively expressed by tanycytes in the adult brain using in situ hybridization histochemistry. We have identified RAX as another gene that demonstrates high and selective expression in the initial data set of our RNA-Seq analysis. Once confirmed (as expected), we will move forward to prepare a transgenic mouse in which Cre-recombinase is selectively produced in Rax-expressing cells under the regulation of tamoxifen.
Results of this work were presented before the annual meeting of The Endocrine Society in June, 2011, and a second paper has been submitted for presentation before annual meeting of The Endocrine Society to be held in June, 2012. Once we have confirmation of the tanycyte transcriptome in our most recent RNA-Seq analysis, the data will be prepared for publication. The work is complete & this is Final Report.
|
| Jobs Created |
0.13 |
| Description of Jobs Created |
Retained: A Post Doctoral fellow is involved in the research proposal, including performance and analysis of microarray data, development of probes for in situ hybridization, tissue processing and interpretation of data. |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0883 |
| Award Information |
| Award Date |
05/08/2009 |
| Award Number |
1R21DK078998-01A2 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$427,588 |
| Funds Invoiced/Received |
$427,588 |
| Expenditure Amount |
$427,588 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
H02.10 |
| Activity Description |
Endocrine, Metabolic & Nutritional Research |
| Sub-Awards Information |
| Sub-awards to Organizations |
1 |
| Sub-award Amounts to Organizations |
$46,599 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
0 |
| Total Amount of payments to vendors greater than $25,000/award |
$0 |
| Number of payments to vendors less than $25,000/award |
292 |
| Total Amount of payments to vendors less than $25,000/award |
$116,895 |
Sub-award 5002632-SERV - MAGYAR TUDOMANYOS AKADEMIA KISERLETI ORVOSTUDOMANYI KUTATOINTEZET
| Sub-Award Amount |
$46,599 |
| Sub-Award Date |
07/17/2009 |
| Sub-Awards Disbursed |
$46,599.00 |
| Project Location - City |
Budapest |
| Project Location - State |
|
| Project Location - Zip Code |
|
| Project Location - Congressional District |
-- |
| Sub-Recipient DUNS Number |
525749540
|
| Sub-Recipient Address |
SZIGONY UTCA 43 |
| Sub-Recipient City |
BUDAPEST |
| Sub-Recipient State |
|
| Sub-Recipient Zip Code |
1083 |
| Sub-Recipient Congressional District |
-- |
Required To Report Top 5
Highly Compensated Officials |
No |
| Location Information |
| Latitude, Longitude |
42º 20' 58",
-71º 3' 49" |
| Congressional District |
08 |
| Address 1 |
800 Washington Street |
| Address 2 |
|
| City |
Boston |
| County |
Suffolk |
| State |
MA |
| Zip |
02111-1526 |
|
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