This grant provided a summer research experience(s) for 9 college students and 1 science educator in health?related scientific research. The college students were: Meixin Wang, Prasant Varghese, Debra Leong, Ranjan Upadhyay, David Shiovitz, Jennifer Wang, Ben Lee, Mitali Rakhit, and Archana Sridhar. The science educator was Robert Rouse. The purpose of the supplement is to accelerate the development of Aims 1.2 and 2 in this STTR project, and at the same time, provide research experience for one science educator and three undergraduate researchers. Robert Rouse helped us screen reagents for the HSV?2 test, determined the coefficient of variation of assay as a function of vacuum pressure, coefficient of variation of assay using different internal standards, and development of integrated reader design. The students also helped quantitate the imprecision of aligning the detection zone to the optics, a potential source of error in achieving an integrated reader with the disposable cassette.