SEATTLE UNIVERSITY
Seattle University (SU) has received an award from the NSF MRI-R2 program to purchase a large-volume preparative ultracentrifuge to support programs in undergraduate research and education. The large-volume preparative ultracentrifuge is initially being used by three different faculty members and undergraduate student researchers to conduct basic research projects. Dr. Stenbak and her students use the ultracentrifuge to investigate the molecular requirements for assembly of foamy viruses (ancient, non-pathogenic retroviruses). Cell fractionation through ultracentrifugation allows students working with Dr. Smith (microbiology/biochemistry) to isolate and characterize bioactive compounds involved in territoriality from Myxococcus sp. Finally, students working with Dr. Murphy (biological chemistry/molecular pharmacology) use the centrifuge to prepare eukaryotic and prokaryotic cell lysates for characterization of Hsp90-based molecular chaperone protein function and activity.
The complete abstract for this award is available in Research.gov at: www.research.gov.
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| AWARD OVERVIEW |
| Award Number |
0960313 |
Funding Agency |
National Science Foundation |
| Total Award Amount |
$127,114 |
Project Location - City |
Seattle |
| Award Date |
12/10/2009 |
Project Location - State |
WA |
| Project Status |
Completed |
Project Location - Zip |
98122-1090
|
| Jobs Reported |
0.00 |
Congressional District |
07 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
SEATTLE UNIVERSITY |
| Recipient DUNS Number |
051241875
|
| Recipient Address |
901 12TH AVE |
| Recipient City |
SEATTLE |
| Recipient State |
Washington |
| Recipient Zip |
98122-4411 |
| Recipient Congressional District |
07 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
MRI-R2: Acquisition of a Large-Volume Preparative Ultracentrifuge for Use in Research and Research Training at Seattle University |
| Project Status |
Completed |
| Final Project Report Submitted |
Yes |
| Project Activities Description |
Biological & Life Sciences |
| Quarterly Activities/Project Description |
The overall goal of this award was to purchase and install a large-volume ultracentrifuge at Seattle University to support faculty research and research training efforts. The ultracentrifuge was successfully acquired and installed and is being used in laboratory classes as well as faculty research. Specific activity in Autumn 2012 involved continued harvesting of virus particles to support undergraduate research projects studying Foamy Virus assembly in the Stenbak lab. During this quarter, continued progress was made analyzing a panel of deletion mutants for defects in viral protein processing and virus assembly. The preliminary results found three different regions within the Foamy virus polymerase protein that appear to be required for either viral cleavage, virus assembly, or both. These mutants are undergoing further characterization in a cell-based bifluorescence assay for protein-protein interaction defects to support the initial findings. Two undergraduate research students participated in this project during the fall quarter and our results were presented at the M.J. Murdock Charitable Trust Conference for Undergraduate Research. These studies have made significant progress and the use of the ultracentrifuge purchased with this grant was, and will continue to be, critical for the success and publication of this work. Equally as important, 6 undergraduate research students and over 30 undergraduate science students had the opportunity to work with and learn from this instrument. Although the grant period is ending, it is clear that students and faculty will be able to utilize this ultracentrifuge for many years to come and this will continue to greatly enhance our research and educational goals at Seattle University. The cost of the equipment was less than the total award amount; therefore the ?Total Federal Amount of ARRA Expenditure? field does not equal the amount in the ?Award Amount? field. No more funds will be expended. |
| Jobs Created |
0.00 |
| Description of Jobs Created |
No jobs were created or retained. |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
49-0101 |
| Award Information |
| Award Date |
12/10/2009 |
| Award Number |
0960313 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
49 |
| Funding Agency Name |
National Science Foundation |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
49 |
| Awarding Agency Name |
National Science Foundation |
| Amount of Award |
$127,114 |
| Funds Invoiced/Received |
$125,603 |
| Expenditure Amount |
$125,603 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
U50 - NTEE |
| Activity Description |
Biological & Life Sciences |
| Sub-Awards Information |
| Sub-awards to Organizations |
0 |
| Sub-award Amounts to Organizations |
$0 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
1 |
| Total Amount of payments to vendors greater than $25,000/award |
$125,603 |
| Number of payments to vendors less than $25,000/award |
1 |
| Total Amount of payments to vendors less than $25,000/award |
$8,180 |
Beckman Coulter, Inc. - Award Number 0960313 - Beckman Coulter, Inc.
| Award Number |
0960313 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
008254708 |
| Vendor HQ Zip Code + 4 |
92835-1091 |
| Vendor Name |
Beckman Coulter, Inc. |
| Product and Service Description |
The vendor provided the Optima L-90K ultracentrifuge along with accompanying rotors and accessories as described in the project awarded. The vendor also provided delivery, installation, and on-site training for use, safety, and maintenance of instrument. |
| Payment Amount |
$125,603 |
| Location Information |
| Latitude, Longitude |
47º 36' 37",
-122º 19' 0" |
| Congressional District |
07 |
| Address 1 |
901 12th Avenue |
| Address 2 |
|
| City |
Seattle |
| County |
King |
| State |
WA |
| Zip |
98122-1090 |
|
 |