UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER AT DALLAS
Inhibition of androgen synthesis is the current treatment of human diseases like prostate
cancer and polycystic ovary syndrome. This approach is suboptimal. By defining how the
enzyme responsible synthesizes these androgens, we hope to establish new
approaches to treatment. Our study will apply to other enzymes that metabolize drugs.
Choose a quarter and click "Go."
| AWARD OVERVIEW |
| Award Number |
1R01GM086596-01A1 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$431,354 |
Project Location - City |
Dallas |
| Award Date |
09/28/2009 |
Project Location - State |
TX |
| Project Status |
Completed |
Project Location - Zip |
75390-9020
|
| Jobs Reported |
0.00 |
Congressional District |
30 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER AT DALLAS |
| Recipient DUNS Number |
800771545
|
| Recipient Address |
5323 HARRY HINES BLVD |
| Recipient City |
DALLAS |
| Recipient State |
Texas |
| Recipient Zip |
75390-7208 |
| Recipient Congressional District |
30 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
Activation of Androgen Biosynthesis and Drug Metabolism by Cytochrome b5 - ARRA Stimulus 2 Yr Resub |
| Project Status |
Completed |
| Final Project Report Submitted |
Yes |
| Project Activities Description |
Medical Specialties Research |
| Quarterly Activities/Project Description |
The final Total Federal Amount of ARRA Expenditure for this project is less than the Award Amount. The project was completed under budget. No more funds will be expended on this project The purposes of this award are: 1) to determine if eliminating cytochrome b5 from the mouse testis by homologous recombination using Cre/loxP technology significantly impairs testosterone synthesis and causes undervirilization of male mice; 2) to determine of the same residues on cytochrome b5 are required for stimulation of various cytochrome P450 activities. Regarding Aim 1, we have repeated the enzyme assay with testicular homogenates, and we have confirmed the defective 17,20-lyase activity in the cytochrome b5 testis knockout animals. We have begun analyses of serum steroids, and while basal testosterone appears normal, the 17-hydroxyprogesterone is markedly elevated. We are planning stimulation experiments and urine steroid analyses to expand these results. Thus, Aim 1 continues to progress well, in part because we have now established the LC-MS/MS assays for the serum steroids, and we are very pleased with the assay?s performance characteristics. I have relocated the laboratory to the University of Michigan, and we have now resumed P450 enzyme expression and assay experiments for Aim 2. I have hired a qualified scientist with specific expertise in cytochrome P450 work for this project. In addition, with Paul Hollenberg in Pharmacology, who is an expert in CYP2E1, we will be better positioned to studies these P450 enzymes other than CYP17A1 with our panel of cytochrome b5 mutations. We have received a no-cost extension, and the grant has been closed out at UT Southwestern in preparation for transfer to my new institution. |
| Jobs Created |
0.00 |
| Description of Jobs Created |
N/A |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0852 |
| Award Information |
| Award Date |
09/28/2009 |
| Award Number |
1R01GM086596-01A1 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$431,354 |
| Funds Invoiced/Received |
$431,347 |
| Expenditure Amount |
$431,347 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
H03.07 |
| Activity Description |
Medical Specialties Research |
| Sub-Awards Information |
| Sub-awards to Organizations |
0 |
| Sub-award Amounts to Organizations |
$0 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
1 |
| Total Amount of payments to vendors greater than $25,000/award |
$42,524 |
| Number of payments to vendors less than $25,000/award |
417 |
| Total Amount of payments to vendors less than $25,000/award |
$46,608 |
AB Sciex LLC - Award Number 1R01GM086596-01A1 - AB Sciex LLC
| Award Number |
1R01GM086596-01A1 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
Not reported |
| Vendor HQ Zip Code + 4 |
60673-1248 |
| Vendor Name |
AB Sciex LLC |
| Product and Service Description |
SERVICE & MAINTENANCE CONTRACTS - FURNISHINGS & EQ |
| Payment Amount |
$42,524 |
| Location Information |
| Latitude, Longitude |
32º 48' 45",
-96º 50' 18" |
| Congressional District |
30 |
| Address 1 |
5323 Harry Hines |
| Address 2 |
|
| City |
Dallas |
| County |
Dallas |
| State |
TX |
| Zip |
75390-9020 |
|
 |