FLORIDA ATLANTIC UNIVERSITY
Natural products represent an excellent source of novel chemical scaffolds that are inherently biologically active. It has been estimated that 78% of the 90 antibacterial agents and 62% of the 79 anticancer agents approved for use in the US over the past two decades have their origins in natural products. With advances in robotics, High Throughput Screening (HTS) has become the primary approach used worldwide to discover new lead compounds for drug development and chemical probes to better understand the disease process. Unfortunately, natural products extracts are not readily amenable for use in discovery programs that use HTS which has led to a significant reduction in opportunities for new drug and chemical probe discovery. The current proposal seeks to overcome this obstacle by creating a unique library of marine natural products (both enriched fractions and purified compounds) that is compatible with HTS and is available to the Life Sciences Research Community as a new research tool.
In this two year project we seek to create a unique library of marine natural products, designated as the Harbor Branch Oceanographic Institute (HBOI) Marine Natural Product Library (MNPL), which will serve as a long term resource to the scientific community. HBOI has been engaged in the production of such a library for the past five years as part of a National Cooperative Drug Discovery Project and has successfully created a library from approximately 150 organisms. Screening of the existing chemical library has allowed for the rapid discovery of many new structures including novel histone deacetylase inhibitors, MCL-1 inhibitors, anti-malarial agents and inhibitors of nuclear factor kappa B. HBOI has a repository of over 14,000 frozen marine specimens and 19,000 marine microbial isolates that could be used in generation of an expanded Marine Natural Products Library. In the current proposal we seek to greatly expand the diversity of the library through adding automation to the fractionation process and hiring new graduates and students to conduct chemical fractionation on up to 750-1250 organisms found in the HBOI Frozen Organism Repository and up to 250-350 organisms from the Marine Microbial Culture Collection. The library will be made available to external collaborators through material transfer agreements, collaborative research agreements and sponsored research projects.
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| AWARD OVERVIEW |
| Award Number |
1RC2AT005917-01 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$1,380,696 |
Project Location - City |
Ft. Pierce |
| Award Date |
09/18/2009 |
Project Location - State |
FL |
| Project Status |
Completed |
Project Location - Zip |
34946-7331
|
| Jobs Reported |
0.00 |
Congressional District |
16 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
FLORIDA ATLANTIC UNIVERSITY |
| Recipient DUNS Number |
004147534
|
| Recipient Address |
777 GLADES RD |
| Recipient City |
BOCA RATON |
| Recipient State |
Florida |
| Recipient Zip |
33431-6424 |
| Recipient Congressional District |
19 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
Creation of a Marine Natural Products Library to Enhance Life Science Research |
| Project Status |
Completed |
| Final Project Report Submitted |
Yes |
| Project Activities Description |
Colleges, Universities, and Professional Schools |
| Quarterly Activities/Project Description |
This project created a library of marine natural products which is a long term resource to the scientific community. The project began in October 2009, was fully up to speed by mid-2010, and was completed in September 2012. The goal of the project was to expand the HBOI marine natural products library and to explore the use of the Waters Autopurification System for automation of natural products purification. Over the course of the project we hired and trained a number of individuals giving them laboratory experience that has led to additional employment opportunities. The Marine Natural Products Library (MNPL) created under this project is accelerating the rate of discovery of both new medicines as well as chemical probes that allow us to better understand the disease process. 6200 fractions were added to the library over the course of the project. Subsets of the library have already been tested with lead molecules identified in assays related to neurodegenerative disease, tuberculosis, cancer, cancer metastasis, inflammation and infectious disease. The library is poised to be assayed in additional assays and based upon these early results the library has turned out to be an exceptional national resource for drug discovery just as we originally proposed. The library is also useful in other fields such as the discovery of new agrochemicals and personal care products and we are beginning to explore these uses. This project met the goals of the ARRA by using a one-time investment to build a resource with lasting return. The project also provided economic stimulus through the creation of new jobs, retaining current employees, creating data that has led to additional funding streams to continue the research, providing training opportunities for new graduates and students and through the purchase of equipment from US scientific equipment manufacturers. The project is 100% complete and we feel that we accomplished all of our proposed specific aims. |
| Jobs Created |
0.00 |
| Description of Jobs Created |
*Copies of signed payroll certifications as well as copies of time cards (if applicable) of all personnel paid from this ARRA funded grant must be submitted This is a summary of hiring over the course of the project:
We hired three new chemistry laboratory technicians (Sandle, Talas, Woods) as full-time employees with benefits. Sandle worked a total of 4269 hours over the course of the project. Talas worked a total of 4462 hours over the course of the project and Wood worked 2924 hours over the course of the project. All three of these technicians had limited expertise in chemical laboratory research when they began their employment but gained substantial experience over the performance period of the grant . Sandle has since taken a permanent job as a chemistry technician in the Orlando area. Her training on this project made her the ideal candidate for this position. Talas and Woods have both gone on to teaching High School Chemistry and are using their experience in the HBOI labs to enrich their teaching programs.
We hired three Summer students (Cammarasana, Paperno and Steckler) to work on the project during the summer college breaks. These individuals were all working towards their college degrees, or planned to return to graduate school and gained laboratory expertise and training working on the project. Cammarasana worked 343 hours over one summer on the microbiology aspects of the project. Paperno worked 840 hours over two summers conducting chemical fractionation and analysis. Steckler worked 533 hours over one summer conducting chemical fractionation and analysis.
We retained four technical staff members (Robertson, Samples, Harmody and Janda) over the course of the project. Robertson worked a total of 651 hours on the chemistry aspects of the project and remains employed in our group. Samples worked a total of 5181 hours on the chemistry aspects of the project and remains employed in our group. Harmody worked a total of 297 hours on the microbiology aspects of the project and remains employed in our group. Janda worked a total of 2173 hours on the microbiology aspects of the project and remains employed in our group.
Dr. Kemami Wangun was retained on the project as a postdoctoral investigator and worked a total of 1588 hours. In 2011, he left HBOI, to pursue permanent career opportunities. Dr. McCarthy was retained on the project and served as co-investigator leading the microbiology aspects of the project. He worked a total of 810 hours on the project. Dr. Wright was retained on the project and served as Principal Investigator- she was paid for a total of 739 hours on the project.
The work conducted by all of these individuals has led to a substantial national resource that is currently providing additional funding streams to allow us to continue to retain all of the retained employees except the Postdoctoral investigator as well as hire new employees to continue the work.
|
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0910 |
| Award Information |
| Award Date |
09/18/2009 |
| Award Number |
1RC2AT005917-01 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$1,380,696 |
| Funds Invoiced/Received |
$1,377,719 |
| Expenditure Amount |
$1,377,719 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
611310 |
| Activity Description |
Colleges, Universities, and Professional Schools |
| Sub-Awards Information |
| Sub-awards to Organizations |
0 |
| Sub-award Amounts to Organizations |
$0 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
4 |
| Total Amount of payments to vendors greater than $25,000/award |
$304,698 |
| Number of payments to vendors less than $25,000/award |
29 |
| Total Amount of payments to vendors less than $25,000/award |
$98,863 |
Beckman Coulter, Inc. - Award Number 1RC2AT005917-01 - Beckman Coulter, Inc.
| Award Number |
1RC2AT005917-01 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
008254708 |
| Vendor HQ Zip Code + 4 |
92835-1091 |
| Vendor Name |
Beckman Coulter, Inc. |
| Product and Service Description |
Biomek FX-P Single Liquid Handler-equipment purchased |
| Payment Amount |
$38,779 |
Genevac Inc. c/o SP Industries - Award Number 1RC2AT005917-01 - Genevac Inc. c/o SP Industries
| Award Number |
1RC2AT005917-01 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
Not reported |
| Vendor HQ Zip Code + 4 |
07101-4830 |
| Vendor Name |
Genevac Inc. c/o SP Industries |
| Product and Service Description |
EZ-2.3 Plus Evaporation System -equipment purchased |
| Payment Amount |
$38,087 |
Teledyne Isco, Inc. - Award Number 1RC2AT005917-01 - Teledyne Isco, Inc.
| Award Number |
1RC2AT005917-01 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
007274194 |
| Vendor HQ Zip Code + 4 |
68504-1328 |
| Vendor Name |
Teledyne Isco, Inc. |
| Product and Service Description |
Rf-200 CombiFlash-equipment purchased |
| Payment Amount |
$38,291 |
WATERS CORPORATION - Award Number 1RC2AT005917-01 - WATERS CORPORATION
| Award Number |
1RC2AT005917-01 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
867704355 |
| Vendor HQ Zip Code + 4 |
01757-3604 |
| Vendor Name |
WATERS CORPORATION |
| Product and Service Description |
Waters Autopurification System-equipment purchased |
| Payment Amount |
$189,540 |
| Location Information |
| Latitude, Longitude |
27º 28' 12",
-80º 19' 50" |
| Congressional District |
16 |
| Address 1 |
5600 U.S. 1 North |
| Address 2 |
|
| City |
Ft. Pierce |
| County |
St. Lucie |
| State |
FL |
| Zip |
34946-7331 |
|
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