TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
In this application, we articulate the vision for a center that will integrate recent computational and experimental advances in cancer systems biology toward the genome-wide prioritization of therapeutic targets using high-throughput screening approaches
Choose a quarter and click "Go."
| AWARD OVERVIEW |
| Award Number |
1RC2CA148308-01 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$4,750,000 |
Project Location - City |
New York |
| Award Date |
09/30/2009 |
Project Location - State |
NY |
| Project Status |
Completed |
Project Location - Zip |
10032-3702
|
| Jobs Reported |
0.32 |
Congressional District |
15 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK |
| Recipient DUNS Number |
621889815
|
| Recipient Address |
630 W 168TH ST FL 4 |
| Recipient City |
NEW YORK |
| Recipient State |
New York |
| Recipient Zip |
10032-3725 |
| Recipient Congressional District |
15 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
Systems Biology of Tumor Progression and Drug-Resistance |
| Project Status |
Completed |
| Final Project Report Submitted |
Yes |
| Project Activities Description |
Medical Research, General/Other |
| Quarterly Activities/Project Description |
Glioblastoma multiforme (GBM): We had previously developed a stable cell line for the signal transducer and activator of transcription 3 (STAT3) luciferase reporter. However after several cell passages, luciferase expression decreased and the STAT3 reporter specificity was lost. This forced us go back and work with a transient reporter system in which the reporter plasmid is transiently transfected into cells and expression measured 48 hours after plasmid transfection. We have optimized the transient transfection conditions, implemented a multiplexed assay for the simultaneous measurement of the STAT3 activity and cell viability. Using this assay we validated the assay with commercially available positive control compounds and then screened 250 compounds previously shown to bind STAT3 through Broad's small molecule array (SMM). We have identifies 31 compounds which decreased STAT3 activity by 20% of control (untreated) level; three of these compounds decrease STAT3 activity by at least 30% of control. We are further characterizing these effects by fine tuned titration studies. Additionally, Dr. Jose Silva is investigating whether the SMM identified STAT3-specific binding compounds reduce STAT3 phosphorylation. T cell acute lymphoblastic leukemia (T-ALL): Based on reviewer comments to a manuscript submitted to Nature, we are currently investigating the mechanism of reversal of glucocorticoid resistance via AKT inhibition in primary patient samples.We are reporting a status of fully completed this quarter and did not utilize all the funds. |
| Jobs Created |
0.32 |
| Description of Jobs Created |
Columbia University has created/retained: Associate Research Scientist, |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0850 |
| Award Information |
| Award Date |
09/30/2009 |
| Award Number |
1RC2CA148308-01 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$4,750,000 |
| Funds Invoiced/Received |
$4,749,677 |
| Expenditure Amount |
$4,749,677 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
H01 |
| Activity Description |
Medical Research, General/Other |
| Sub-Awards Information |
| Sub-awards to Organizations |
0 |
| Sub-award Amounts to Organizations |
$0 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
3 |
| Total Amount of payments to vendors greater than $25,000/award |
$214,609 |
| Number of payments to vendors less than $25,000/award |
901 |
| Total Amount of payments to vendors less than $25,000/award |
$1,102,036 |
Dharmacon, Inc. - Award Number 1RC2CA148308-01 - Dharmacon, Inc.
| Award Number |
1RC2CA148308-01 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
884350976 |
| Vendor HQ Zip Code + 4 |
80026-3375 |
| Vendor Name |
Dharmacon, Inc. |
| Product and Service Description |
siRNA library |
| Payment Amount |
$140,006 |
LONZA WALKERSVILLE, INC. - Award Number 1RC2CA148308-01 - LONZA WALKERSVILLE, INC.
| Award Number |
1RC2CA148308-01 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
609095955 |
| Vendor HQ Zip Code + 4 |
21793-8415 |
| Vendor Name |
LONZA WALKERSVILLE, INC. |
| Product and Service Description |
Normal human cells, media, serum, assay kits, agarose, gels, stains & markers. Endotoxin detection products, Transfection products. |
| Payment Amount |
$38,484 |
QIAGEN INC. - Award Number 1RC2CA148308-01 - QIAGEN INC.
| Award Number |
1RC2CA148308-01 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
197294564 |
| Vendor HQ Zip Code + 4 |
91355-1018 |
| Vendor Name |
QIAGEN INC. |
| Product and Service Description |
LiquiChip Workstation IS 200 |
| Payment Amount |
$36,119 |
| Location Information |
| Latitude, Longitude |
40º 50' 28",
-73º 56' 25" |
| Congressional District |
15 |
| Address 1 |
|
| Address 2 |
|
| City |
New York |
| County |
New York |
| State |
NY |
| Zip |
10032-3702 |
|
 |