UNIVERSITY OF MASSACHUSETTS
This project directly addressed the broad Challenge Area (06) Enabling Technologies and specific Challenge Topic: Chemist/Biologist collaborations facilitating tool development. The goal of this project is to target the N linked glycans on ion-conducting and regulatory subunits of K+ channels found in cardiomyocytes. There are three aims of this project. In Aim 1, the Kobertz laboratory will chemically-derivatize peptide toxins such that when they bind to their cognate K+ channel complex they will covalently label the N-linked glycans on the proteins in the complex with a chemical probe. The labeling efficiency and specificity of the chemical reactions will be determined in standard laboratory cell lines. Aims 2 and 3 (Colecraft Laboratory, Columbia Medical School) will demonstrate the utility of the panel of derivatized toxins by identifying the native regulatory subunits co assembled with cardiac K+ channels and determining the cell surface localization and turnover of K+ channels in living cardiomyocytes.
| AWARD OVERVIEW |
| Award Number |
1RC1HL099759-01 |
Funding Agency |
Department of Health and Human Services |
| Total Award Amount |
$999,154 |
Project Location - City |
Worcester |
| Award Date |
09/21/2009 |
Project Location - State |
MA |
| Project Status |
Completed |
Project Location - Zip |
01655-0002
|
| Jobs Reported |
3.02 |
Congressional District |
03 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
UNIVERSITY OF MASSACHUSETTS |
| Recipient DUNS Number |
603847393
|
| Recipient Address |
55 LAKE AVE N |
| Recipient City |
WORCESTER |
| Recipient State |
Massachusetts |
| Recipient Zip |
01655-0002 |
| Recipient Congressional District |
03 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
Chemical Tools for Profiling Functioning Ion channel complexes |
| Project Status |
Completed |
| Final Project Report Submitted |
Yes |
| Project Activities Description |
Medical Research, General/Other |
| Quarterly Activities/Project Description |
In the last quarter, we published a paper based on the toxin-delivery agents to selectively control K+ channel function using unnatural sugars: Hua, Z.; et al. Bioorg. Med. Chem. Lett. (2011), doi:10.1016/j.bmcl.2011.04.099 With regard to the potassium-sensitive fluorophore, we have made excellent progress. We have synthesized the bottom half of the cryptand and have made the bis-acid chloride to complete the synthesis of the potassium-binding moiety. In addition, we have begun the synthesis of the fluorescent dye, which will be attached to the potassium-binding cryptand upon its synthetic completion. In terms of scale, we have over 30g of the cryptand so we should be able to complete the synthesis with enough material to perform over 100 experiments. Similarly, we have enough thiol-sugar to metabolically-label the different cells with the potassium-sensitive fluorophore. There is 1 subrecipient payment pending and once processed the funds will be fully invoiced |
| Jobs Created |
3.02 |
| Description of Jobs Created |
2 Assoc Professor, Post Doc Assoc, Research Associate, UMass Graduate Student, 2 Assoc Research Scientists |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
75-0871 |
| Award Information |
| Award Date |
09/21/2009 |
| Award Number |
1RC1HL099759-01 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
75 |
| Funding Agency Name |
Department of Health and Human Services |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
75 |
| Awarding Agency Name |
Department of Health and Human Services |
| Amount of Award |
$999,154 |
| Funds Invoiced/Received |
$907,710 |
| Expenditure Amount |
$907,710 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
H01 |
| Activity Description |
Medical Research, General/Other |
| Sub-Awards Information |
| Sub-awards to Organizations |
1 |
| Sub-award Amounts to Organizations |
$399,348 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
0 |
| Total Amount of payments to vendors greater than $25,000/award |
$0 |
| Number of payments to vendors less than $25,000/award |
154 |
| Total Amount of payments to vendors less than $25,000/award |
$40,891 |
Sub-award 0006114403 - TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
| Sub-Award Amount |
$399,348 |
| Sub-Award Date |
10/28/2009 |
| Sub-Awards Disbursed |
$307,926.46 |
| Project Location - City |
NEW YORK |
| Project Location - State |
NY |
| Project Location - Zip Code |
10032-3702 |
| Project Location - Congressional District |
15 |
| Sub-Recipient DUNS Number |
621889815
|
| Sub-Recipient Address |
630 W 168TH ST FL 4 |
| Sub-Recipient City |
NEW YORK |
| Sub-Recipient State |
New York |
| Sub-Recipient Zip Code |
10032-3725 |
| Sub-Recipient Congressional District |
15 |
Required To Report Top 5
Highly Compensated Officials |
No |
| Location Information |
| Latitude, Longitude |
42º 16' 39",
-71º 45' 33" |
| Congressional District |
03 |
| Address 1 |
55 Lake Avenue North |
| Address 2 |
|
| City |
Worcester |
| County |
Worcester |
| State |
MA |
| Zip |
01655-0002 |
|
 |