REGENTS OF THE UNIVERSITY OF MICHIGAN
This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).
This Major Research Instrumentation-Recovery and Reinvestment (MRI-R2) award funds the development of two versatile, modular fluorescence microscopy platforms for tracking and mechanically probing single molecules/particles at the University of Michigan (UM), and the installation of a third, user-friendly microscope system for user training and project development. The development and application of two open-access single molecule tracking and probing microscopes transforms the limited capability for high-content screening of biological samples. Benchmarks are set that make both instruments operational within two years and lay out plans for testing, further optimization and expansion over an additional two years, after which recharges will maintain them. The instruments are being utilized for research and education by the University of Michigan community and beyond, engaging physical and biological scientists as well as an engineer. This development activity helps establish a unique, nation-wide, educational Center of Single Molecule Analysis (CoSiMA) in a highly cross-disciplinary, emerging field of transformative power in the biosciences. CoSiMA is engaging in vigorous outreach efforts to experimentalists, theorists, undergraduates, and high school students with large, local, underrepresented groups, as well as the public at large, in close collaboration with UM's existing IDEA Institute. These efforts are facilitated by the creation of a central laboratory facility with access to multiple single molecule microscopes under guidance of a Steering Committee and a senior staff scientist, as well as by the broad inspirational appeal of the emerging single molecule biosciences. Results from the studies enabled by the new equipment are disseminated by student and faculty presentations at regional and national meetings, and through publication in peer-reviewed journals.
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| AWARD OVERVIEW |
| Award Number |
0959823 |
Funding Agency |
National Science Foundation |
| Total Award Amount |
$1,163,026 |
Project Location - City |
Ann Arbor |
| Award Date |
02/19/2010 |
Project Location - State |
MI |
| Project Status |
More than 50% Completed |
Project Location - Zip |
48109-1271
|
| Jobs Reported |
1.86 |
Congressional District |
12 |
| Project Location - Country |
US |
|
|
Recipient Information
(Grants)
| Recipient Information (Grants) |
|
Recipient Name
|
REGENTS OF THE UNIVERSITY OF MICHIGAN |
| Recipient DUNS Number |
073133571
|
| Recipient Address |
503 THOMPSON ST |
| Recipient City |
ANN ARBOR |
| Recipient State |
Michigan |
| Recipient Zip |
48109-1340 |
| Recipient Congressional District |
12 |
| Recipient Country |
USA |
Required to Report Top 5
Highly Compensated Officials |
No |
Projects and Jobs Information
| Projects and Jobs Information |
| Project Title |
MRI-R2: Development of High-Resolution Single Fluorescent Particle Tracker and Nanomanipulator |
| Project Status |
More than 50% Completed |
| Final Project Report Submitted |
No |
| Project Activities Description |
Chemistry |
| Quarterly Activities/Project Description |
We are upgrading the existing confocal microscope to perform pulsed interleaved excitation (PIE). The supercontinuum laser will be spectrally split into two wavelengths using a dichroic. The longer wavelength will be delayed by 25 ns and the laser is pulsed at a frequency of 20 MHz (50 ns between pulses), which will result in alternating excitation pulses. Optics and hardware required for this are being purchased and assembled. Two graduate students from Biophysics, one undergraduate student from Chemistry and a postdoctoral fellow from the Medical school were added to the users’ roster. The graduate students and the undergraduate are independent, the postdoc’s experiments are performed by SMART center staff.
We have purchased an astigmatic lens and a lens holder/switch to implement 3-dimensional imaging on the Single particle tracker. Axial localization is adjudicated based on the intensity profile of the sample under investigation – particles in focus have evenly distributed radial intensity profiles whereas particles just above or below the focal plane have elliptical/ellipsoidal intensity profiles elongated along different major axes. Two new users were added to the roster; both are postdoctoral fellows from the Medical school. One user is independent, while the other postdoc’s experiments are performed by SMART center staff.
An Environmental Cell was purchased for the TT-AFM to scan samples in liquids/buffer. Previously, samples were scanned in air, a non-native environment for biological macromolecules. Two new users from Chemistry were trained on the AFM by SMART center staff.
An additional (third) postdoc was hired at 50% to complement the existing 100% and 50% postdocs and complete the assembly of the Single Particle Nanomanipulator. Final optical, hardware and software parts of the assembly are currently being purchased. Final assembly and initial calibration of the system are expected to begin in early April. |
| Jobs Created |
1.86 |
| Description of Jobs Created |
Research Assoicate I, Research Fellow |
Purchaser Information
(Grants)
| Purchaser Information |
| Contracting Office ID |
Not Reported |
| Contracting Office Name |
Not Available |
| Contracting Office Region |
Not Available |
| TAS Major Program |
49-0101 |
| Award Information |
| Award Date |
02/19/2010 |
| Award Number |
0959823 |
| Order Number |
|
| Award Type |
Grants |
| Funding Agency ID |
49 |
| Funding Agency Name |
National Science Foundation |
| Funding Office Name |
Not Available |
| Awarding Agency ID |
49 |
| Awarding Agency Name |
National Science Foundation |
| Amount of Award |
$1,163,026 |
| Funds Invoiced/Received |
$1,078,565 |
| Expenditure Amount |
$1,078,565 |
| Infrastructure Expenditure Amount |
$0 |
| Infrastructure Purpose and Rationale |
Not Reported |
| Infrastructure Point of Contact Name |
Not Reported |
| Infrastructure Point of Contact Email |
Not Reported |
| Infrastructure Point of Contact Phone |
Not Reported |
| Infrastructure Point of Contact Address |
Not Reported |
| Infrastructure Point of Contact City |
Not Reported |
| Infrastructure Point of Contact State |
Not Reported |
| Infrastructure Point of Contact Zip |
Not Reported |
Product or Service Information
(Grants)
| Product or Service Information |
| Primary Activity Code |
U04.04 |
| Activity Description |
Chemistry |
| Sub-Awards Information |
| Sub-awards to Organizations |
0 |
| Sub-award Amounts to Organizations |
$0 |
| Sub-Awards to Individuals |
0 |
| Sub-Award Amounts to Individuals |
$0 |
| Number of Sub-awards less than $25,000/award |
0 |
| Amount of Sub-awards less than $25,000/award |
$0 |
| Number of payments to vendors greater than $25,000 |
8 |
| Total Amount of payments to vendors greater than $25,000/award |
$424,720 |
| Number of payments to vendors less than $25,000/award |
152 |
| Total Amount of payments to vendors less than $25,000/award |
$196,698 |
AFM Workshop - Award Number 0959823 - AFM Workshop
| Award Number |
0959823 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
Not reported |
| Vendor HQ Zip Code + 4 |
90755-5200 |
| Vendor Name |
AFM Workshop |
| Product and Service Description |
Kit of the TT AFM, 50 Micron XYZ Scanner for the TT AFM, TT AFM kit constuction workshop, Vibrating mode probes QTY 15, Control computer/monitor for workshop AFM products |
| Payment Amount |
$26,910 |
Bitplane Inc - Award Number 0959823 - Bitplane Inc
| Award Number |
0959823 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
084032833 |
| Vendor HQ Zip Code + 4 |
55104-4945 |
| Vendor Name |
Bitplane Inc |
| Product and Service Description |
Imaris 7.5 Confocal Standard Package, Amaris Track 7.5, ImarisXT 7.5 - Programming Interface, AutoQuant X v.3-CWF |
| Payment Amount |
$33,153 |
Coherent, Inc. - Award Number 0959823 - Coherent, Inc.
| Award Number |
0959823 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
043719210 |
| Vendor HQ Zip Code + 4 |
95054-1112 |
| Vendor Name |
Coherent, Inc. |
| Product and Service Description |
Saphire Laser System |
| Payment Amount |
$38,023 |
Eppendorf North America, Inc. - Award Number 0959823 - Eppendorf North America, Inc.
| Award Number |
0959823 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
152088142 |
| Vendor HQ Zip Code + 4 |
11590-2826 |
| Vendor Name |
Eppendorf North America, Inc. |
| Product and Service Description |
Injectman N12 Micromanip, FemtoJet Microinjector, Olympus IX50/70 Adapter, Femtotips, Microloaders |
| Payment Amount |
$27,764 |
Olympus America Inc - Award Number 0959823 - Olympus America Inc
| Award Number |
0959823 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
017018859 |
| Vendor HQ Zip Code + 4 |
18034-8229 |
| Vendor Name |
Olympus America Inc |
| Product and Service Description |
2 IXON ULTRA. TUCAM. CFP-RFP. 1X ADAPT; IXON ULTRA897 EMCCD |
| Payment Amount |
$104,517 |
Olympus America Inc - Award Number 0959823 - Olympus America Inc
| Award Number |
0959823 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
017018859 |
| Vendor HQ Zip Code + 4 |
18034-8229 |
| Vendor Name |
Olympus America Inc |
| Product and Service Description |
Celltirf Laser System |
| Payment Amount |
$54,664 |
Olympus America Inc - Award Number 0959823 - Olympus America Inc
| Award Number |
0959823 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
017018859 |
| Vendor HQ Zip Code + 4 |
18034-8229 |
| Vendor Name |
Olympus America Inc |
| Product and Service Description |
Microscope |
| Payment Amount |
$69,689 |
PI (PHYSIK INSTRUMENTE) L.P. - Award Number 0959823 - PI (PHYSIK INSTRUMENTE) L.P.
| Award Number |
0959823 |
| Sub-Award Number |
N/A |
| Vendor DUNS Number |
142559447 |
| Vendor HQ Zip Code + 4 |
01501-1304 |
| Vendor Name |
PI (PHYSIK INSTRUMENTE) L.P. |
| Product and Service Description |
Nanopositioning System and Slide size aperture |
| Payment Amount |
$70,000 |
| Location Information |
| Latitude, Longitude |
42º 17' 16",
-83º 43' 11" |
| Congressional District |
12 |
| Address 1 |
3003 South State St. |
| Address 2 |
Room 1062 |
| City |
Ann Arbor |
| County |
Washtenaw |
| State |
MI |
| Zip |
48109-1271 |
|
 |